Whole Genome Amplification - Methods and Protocols

od Springer US
Stan: Nowy
569,93 zł
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Springer US Whole Genome Amplification - Methods and Protocols
Springer US - Whole Genome Amplification - Methods and Protocols

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Dostawa: między wtorek, 31 maja 2022 a czwartek, 2 czerwca 2022
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This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration and will help researchers, both new and experienced, yield successful results.

Dalsza informacja

Adnotacja do ilustracji:
X, 284 p. 18 illus., 13 illus. in color.
Spis treści:

1. Principles of Whole Genome Amplification

Zbigniew Tadeusz Czyz, Stefab Kirsch, and Bernhard Polzer

2. Bias in Whole Genome Amplification: Causes and Considerations

Jeffrey X. Sabina and John H. Leamon

3. The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis

Roland Kirchner and Marianna Alunni-Fabbroni

4. Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole Genome Amplification: An Overview

Joost F. Swennenhuis and Leon W.M.M. Terstappen

5. Deterministic Whole Genome Amplification of Single Cells

Zbigniew T. Czyz and Christoph A. Klein

6. Construction of a DNA Library on Microbeads Using Whole Genome Amplification

Kojima T., Zhu B., and Nakano H.

7. Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4)

Amin El-Heliebi, Shukun Chen, and Thomas Kroneis

8. Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase

Thomas Kroneis and Amin El-Heliebi

9. Using Multiplex-PCR for Assessing the Quality of Whole Genome Amplified DNA

Amin El-Heliebi, Shukun Chen, and Thomas Kroneis

10. Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis

Thomas Kroneis and Amin El-Heliebi

11. Laser-Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1

Zbigniew T. Czyz, Nikolas H. Stoecklein, and Bernhard Polzer

12. Whole Genome Amplification from Blood Spot Samples

Karina Meden


13. Analysis of Whole Mitogenomes from Ancient Samples

Gloria G. Fortes and Johanna L.A. Paijmans

14. Copy Number

Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification

Eftychia Dimitriadou, Masoud Zamani Esteki, and Joris Robert Vermeesch

15. Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells

Christin Gasch, Klaus Pantel, and Sabine Riethdorf

16. Whole Genome Amplification of Labeled Viable Single Cells Suited for Array Comparative Genomic Hybridization

Thomas Kroneis and Amin El-Heliebi

17. Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses

Thomas Kroneis, Shukun Chen, and Amin El-Heliebi

18. Detection and Characterization of Circulating Tumor Cells by the CellSearch Approach

Frank Coumans and Leon W.M.M. Terstappen

Kroneis, Thomas;Kroneis
Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Twarda oprawa
Springer US
DI Dr. Thomas Kroneis Medical University Graz Institute of Cell Biology, Histology & Embryology Head of Research Unit for Single Cell Analysis +43(0)316/380-4236 mailto: thomas.kroneis@medunigraz.at
1st ed. 2015
Liczba stron:

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569,93 zł
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