Ewing Sarcoma - Methods and Protocols

od Springer US
Stan: Nowy
509,94 zł
Zawiera podatek VAT, darmowa dostawa
Springer US Ewing Sarcoma - Methods and Protocols
Springer US - Ewing Sarcoma - Methods and Protocols

Podoba Ci się ten produkt? Przekaż dalej!

509,94 zł zawiera VAT
Dostępnych sztuk: 1 Dostępnych tylko 1 sztuk
Dostawa: między wtorek, 31 maja 2022 a czwartek, 2 czerwca 2022
Sprzedaż i wysyłka: Dodax

Sprawdź inne opcje zakupu

1 oferta za 595,27 zł

Sprzedaje Dodax

595,27 zł zawiera VAT
Stan: Nowy
Darmowa dostawa
Dostawa: między wtorek, 31 maja 2022 a czwartek, 2 czerwca 2022
Pokaż inne opcje zakupu

Opis

This volume aims at providing a selection of the most effective and widely employed protocols used in the Ewing sarcoma field. Chapters are divided into five sections detailing the molecular biology techniques used to analyze Ewing sarcoma including tissue preservation and methods used in morpho-molecular diagnostics and routine immune-histological analysis. Additional wet-lab protocols comprise in vitro techniques including re-expression and functional analyses of  target genes together with in vivo tools and models such as patient-derived xenografts (PDX) and orthotopic xenografts for metastasis assessment in Ewing sarcoma. A full section on bioinformatic approaches to Ewing sarcoma covering ChIPseq, epigenetic and systems biology analyses finalizes this volume. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


 


Cutting-edge and clearly written, Ewing sarcoma: Methods and Protocols aims to provide methods to further the study of Ewing sarcoma and bring researchers one step forward in the quest to fight this devastating disease.

Dalsza informacja

Adnotacja do ilustracji:
7 SW-Abb., 50 Farbabb.
Język:
Angielski
Spis treści:

Part I: Introduction



1. Germline Variation and Somatic Alterations in Ewing Sarcoma



Mitchell J. Machiela and Thomas G. P. Grünewald



 



2. Western Blot Analysis in Ewing Sarcoma



Aruna Marchetto and Laura Romero-Pérez



 



Part II: Molecular Biology Techniques Applied To Ewing Sarcoma



3. Tissue Preservation and FFPE Samples: Optimized Nucleic Acids Isolation in Ewing Sarcoma



Laura Romero-Pérez and Thomas G. P. Grünewald



 



4. Liquid Biopsies in Ewing Sarcoma



Manuela Krumbholz and Markus Metzler



 



5. (Immuno-)histological Analysis of Ewing Sarcoma



David Marcilla, Isidro Machado, Thomas G. P. Grünewald, Antonio Llombart-Bosch, and Enrique de Álava




 



6. Molecular Approaches to Diagnosis In Ewing Sarcoma: Fluorescence in situ hybridization (FISH)



Marcel Trautmann and Wolfgang Hartmann



 



7. Molecular Approaches to Diagnosis in Ewing Sarcoma: RT-PCR



Carlos Rodríguez-Martín and Javier Alonso




 



8. Molecular Approaches to Diagnosis in Ewing Sarcoma: Targeted RNA Sequencing



Carmen Salguero-Aranda and Juan Diaz-Martin




 



Part III: Diagnostics in Ewing Sarcoma



9. Ewing-specific (re)expression Models



Maximilian M. L. Knott and Florencia Cidre-Aranaz



 



10. Analysis of Regulatory Sequences by Dual-luciferase Reporter Assays in Ewing Sarcoma



Tilman L. B. Hölting and Maximilian M. L. Knott




 



Part IV: Cellular biology and cell culture techniques



11. Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma



Cornelius Funk and Julian Musa



 



12. Drug Screening by Resazurin Colorimetry in Ewing Sarcoma



Julian Musa and Florencia Cidre-Aranaz




 



13. Analysis of Migration and Invasion in Ewing Sarcoma



Florencia Cidre-Aranaz




 



Part V: Animal models in Ewing sarcoma



14. Genetically Engineered Mouse Model in Ewing Sarcoma



Miwa Tanaka and Takuro Nakamura



 



15. Tumor Growth Analysis of Ewing Sarcoma Cell Lines Using Subcutaneous Xenografts in Mice



Florencia Cidre-Aranaz and Shunya Ohmura



 



16. Metastasis Assessment in Ewing Sarcoma Using Orthotopic Xenografts



Roser López-Alemany and Oscar M. Tirado




 



17. Orthotopic Implants in Mice



Elizabeth Stewart



 



18. Ewing Sarcoma PDX Models



D. Surdez, L. Landuzzi, K. Scotlandi, and M.C. Manara



 



Part VI: Bioinformatic approaches to Ewing sarcoma 19. Using Zebrafish Larvae as a Xenotransplantation Model to Study Ewing Sarcoma

Susana Pascoal, Sarah Grissenberger, Eva Scheuringer, Rita Fior, Miguel Godinho Ferreira, and  Martin Distel



 



20. Main Repositories and Databases used in Ewing Sarcoma Research



Maria Debiec-Rychter



 



21. Chromatin Immunoprecipitation Followed by Next-Generation Sequencing (ChIP-seq) Analysis in Ewing Sarcoma         



Gwenneg Kerdivel and Valentina Boeva



 



22. Epigenetic Analysis in Ewing Sarcoma



Jeremy M. Simon and Nicholas C. Gomez



 



23. Systems Biology Analysis for Ewing Sarcoma



Marianyela Petrizzelli, Jane Merlevede, and Andrei Zinovyev

Edycja:
1st ed. 2021
Liczba stron:
337
Redaktor:
Cidre-Aranaz, Florencia;Cidre-Aranaz
G. P. Grünewald, Thomas;G. P. Grünewald
Uwagi:
Includes cutting-edge methods and protocols


Provides step-by-step detail essential for reproducible results


Contains key notes and implementation advice from the experts

Nośnik:
Miękka oprawa
Publisher:
Springer US

Dane podstawowe

Rodzaj produktu:
Książka z miękką oprawą
Wymiary opakowania:
0.254 x 0.178 x 0.021 m;
GTIN:
09781071610220
DUIN:
VA0AN4EIA8L
509,94 zł
Na naszej stronie wykorzystujemy pliki cookies, aby strona była jeszcze bardziej funkcjonalna i przyjazna użytkownikowi. Prosimy kliknąć „Akceptuj cookies”! Więcej informacji znajdziesz w Polityce prywatności.